Indonesia Conference Directory

Corresponding Author
Wibowo Budi Prasetyo

Institutions
1. Undergraduate Program Medical Doctor, Faculty of Medicine, Universitas Padjadjaran, Jatinangor 45363, Indonesia.
2. Physiology Division, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Jatinangor 45363, Indonesia.
3. Physiology Molecular Laboratory, Biological Activity Division, Central Laboratory, Universitas Padjadjaran, Jatinangor 45363, Indonesia.
4. Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia.

Abstract
Exercise-induced hearth muscle adaptation for under stress conditions, known as Mitochondria Quality Control. Each stage of Mitochondria quality control, has a coactivator as a trigger for the process. Where it starts from biogenesis with PGC-1α , After that, it will enter the fusion-fission cycle stage which are mediated by Mfn1 and Mfn2 for fusion in the outer membrane and Opa1 for fusion in the inner membrane. After fusion, fission occurs in the old and unhealthy parts mediated by Drp1. Finally, the old and unhealthy mitochondrial section occurs mitophagy mediated by PINK-PARKIN signaling pathway. The aim of this study was to determine the difference effect of PGC-1α, Mfn1, Mfn2, Opa1, Drp1, PINK, and Parkin expression in aerobic physical activity for Mitochondria Quality Control in cardiac muscle. This is limited because only rats are used. 12 male wistar rats were assigned to 2 groups: sedentary control and Aerobic-intensity (AE, 15m/minute). Training was conducted 30 minutes/day with a 5 times/week interval for 8 weeks. On the last day, control and exercise groups were taken blood serum from the retro-orbital sinus for culture of H9C2 Cell line, and extract RNA and protein. The expression of the PGC-1α, Mfn1, Mfn2, Opa1, Drp1, PINK, and Parkin gene in cardiac muscles was then amplified using reversed PCR and detected by agarose gel electrophoresis. This study concludes that different effect of PGC-1α, Mfn1, Mfn2, Opa1, Drp1, PINK, and Parkin gene expression level were increased level affected by aerobic physical activity in cardiac muscle.

Keywords
Aerobic Exercise, Mitochondrial Quality Control, H9C2 Cell Line PGC-1α, Mfn1, Mfn2, Opa1, Drp1, PINK1, Parkin

Topic
Biomarker, Drug Design, Delivery and Teragnostic

Link: https://ifory.id/abstract/Xztm4cTvHnbZ

Corresponding Author
Bela Ita Karina

Institutions
Padjadjaran University

Abstract
Exercise is one of dementia-s modifiable factors. Exercise could delay neurodegeneration process by increase Klotho, endogen antioxidant and known as anti-aging gene in human. Klotho in hippocampus have certain function such as neuroprotective against oxidative damage induced by β amyloid and glutamate , regulate hippocampal synaptic plasticity, and enhance GluN2B concentration. However, there-s no study about optimal duration of exercise to enhance Klotho and GluN2B expression in hippocampus. This study conducted to compare acute and chronic exercise effect on hippocampal klotho and GluN2B in Rat This study aims to analyse the effect of acute and chronic treadmill exercise on Klotho and GluN2B gene expression in rat-s hippocampus. This study was done using semi-quantitative and animal experimental study. This study used 36 wistar rat from Biofarma were divided into 4 group, 2 control group (acute control and chronic control) and 2 exercise group (Acute and chronic). All group received moderate intensity(20m/min) in 30min/day for 5x/week. After treatment was given, rats were terminated under anaesthesia, hippocampus was extracted from brain, and expression of klotho and GluN2B were studied using PCR and Electrophoresis. Gel band of each gene was visualized using BioDocAnalyze and band thickness quantified using ImageJ. Statistical analysis were done using IBM SPSS version 20.0 software. Data were served as means ± standard error of means. Data were statistically analyzed with Multivariate Analysis of Variance (MANOVA) followed by Post Hoc comparison using Tukey and Dunett. P-Values ˂ 0.05 were considered to be significant.

Keywords
Klotho, GluN2B, exercise, duration

Topic
Biomarker, Drug Design, Delivery and Teragnostic

Link: https://ifory.id/abstract/AWJxTh3YemUN

Corresponding Author
Meutia Fakhriah Basuki

Institutions
Fakultas Teknik Sipil dan Lingkungan, Institut Teknologi Bandung

Abstract
Seiring dengan pertumbuhan industri kecil menengah (IKM) yang pesat, hal ini berbandingan lurus dengan dampak yang ditimbulkan terhadap lingkungan dan lingkungan kerja tersebut. Industri batik termasuk salah satu IKM yang menjadi unggulan di beberapa daerah Indonesia, seperti Cirebon dan Yogyakarta. Dampak yang ditimbulkan pada lingkungan berasal dari bahan produksi batik yang dapat mencemari udara ambien dan udara di lingkungan kerja. Sebagian besar industri batik di Cirebon dan Yogyakarta beroperasi dalam skala home industry, yang tidak memiliki pengolahan limbah udara. Dampak yang ditimbulkan tidak hanya emisi namun juga pada pekerja yang terinhalasi udara tersebut. Pada salah satu Industri Batik di Cirebon ditemukan partikulat dan gas hidrokarbon BTEX dan PM 2,5 dan logam berat pada partikulat di dalam ruang kerja pengrajin batik. Pengukuran udara di ruang kerja pengrajin batik digunakan Low Volume Sampler dengan flow 2,5 L/min. Dari hasil pengukuran dapat diidentifikasi kandungan PM 2,5 di ruang kerja pengrajin batik pada kisaran 3,53 – 109,14 μg/m3. Terdapat pula kandungan toluene pada kisaran sebesar 0,259 – 0,288 mg/m3 dan 0,123 – 0,128 mg/m3 xilen. Logam berat yang terkandung dalam partikulat antara lain Al, Zn, dan Fe. Dari hasil tersebut ditemukan konsentrasi PM 2,5 yang melebihi ambang batas standar WHO yaitu 25 μg/m3. Maka untuk mengurangi dampak dari limbah udara di lingkungan kerja diperlukan sistem pembuangan lokal di Industri Batik yang termasuk dalam pendekatan produksi bersih.

Keywords
pencemaran udara, BTEX, produksi bersih, batik, exhaust-ventilation

Topic
Biomarker, Drug Design, Delivery and Teragnostic

Link: https://ifory.id/abstract/h4TaHdCuJ2pv